ABSTRACT:
Fluoro-Ruby is a fluorescently labeled low molecular weight dextran that can be used for the in vivo localization of efferent axonal projections of neurons of interest. Fluoro-Ruby is typically pressure injected as a 10% solution in water or saline. Common injection volumes are in the range of 0.1 – 0.25 ul, delivered over a 10 min interval. Survival intervals are typically in the range of 1-10 days post injection. Occasionally Fluoro-Ruby is used for the localization of vascular pericytes. In such studies, 1 ul of 10% Fluoro-Ruby is pressure injected into the third ventricle of the brain. Following a 1-2 day survival interval, all brain perivascular pericytes will be labeled.

Compound: Fluoro-Ruby

Classification: Tetramethylrhodamine dextran-amine, 10,000 molecular weight

Appearance: Pink to crimson colored powder

Solubility: Highly soluble in aqueous solutions

Purity: Thin layer chromatography using cellulose plates and a solvent system of acetonitrile and water (6:4) revealed no detectable free TRITC dye.

Substitution: 1 molecule TRITC per molecule of dextran-amine

Average Molecular Weight: 10,500

Excitation Peak: 540 nm

Emission Peak: 600 nm

Filter System for Visualizing: Standard TRITC filter

Storage: The dry powder can be stored at room temperature or refrigerated. Solutions should be refrigerated when not in use.

Toxicity: Although the compound appears to be of low toxicity, it has not been extensively evaluated and therefore routine laboratory caution should be exercised. Not approved for human consumption.

REFERENCE:
Schmued, L., Kyriakidis, K., & Heimer, L., In vivo anterograde and retrograde axonal transport of the fluorescent rhodamine-dextran-amine, Fluoro-Ruby, within the CNS. Brain Res, 526 (1990) 127-134.

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