Fluoro-Ruby is a fluorescently labeled low molecular weight dextran that can be used for the in vivo localization of efferent axonal projections of neurons of interest. Fluoro-Ruby is typically pressure injected as a 10% solution in water or saline. Common injection volumes are in the range of 0.1 – 0.25 ul, delivered over a 10 min interval. Survival intervals are typically in the range of 1-10 days post injection. Occasionally Fluoro-Ruby is used for the localization of vascular pericytes. In such studies, 1 ul of 10% Fluoro-Ruby is pressure injected into the third ventricle of the brain. Following a 1-2 day survival interval, all brain perivascular pericytes will be labeled.
Classification: Tetramethylrhodamine dextran-amine, 10,000 molecular weight
Appearance: Pink to crimson colored powder
Solubility: Highly soluble in aqueous solutions
Purity: Thin layer chromatography using cellulose plates and a solvent system of acetonitrile and water (6:4) revealed no detectable free TRITC dye.
Substitution: 1 molecule TRITC per molecule of dextran-amine
Average Molecular Weight: 10,500
Excitation Peak: 540 nm
Emission Peak: 600 nm
Filter System for Visualizing: Standard TRITC filter
Storage: The dry powder can be stored at room temperature or refrigerated. Solutions should be refrigerated when not in use.
Toxicity: Although the compound appears to be of low toxicity, it has not been extensively evaluated and therefore routine laboratory caution should be exercised. Not approved for human consumption.
Schmued, L., Kyriakidis, K., & Heimer, L., In vivo anterograde and retrograde axonal transport of the fluorescent rhodamine-dextran-amine, Fluoro-Ruby, within the CNS. Brain Res, 526 (1990) 127-134.
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