New & Future Dyes

Recently Developed

Euro-Glo ®

This tracer will label both myelinated fibers and amyloid plaques. Although it has been available for over a year, it has not been extensively used, possibly because it differs from most of the other tracers that target A-beta aggregates. By contrast, Euro-Glo is thought to label glycolipids, such as gangliosides, resulting in a different labeling pattern. The relatively long staining times (5-7 days) required may be less convenient than more rapid stains such as Amylo-Glo. This compound represents the only histochemical stain for use labeling brain tissue sections whose fluorescence is based on the presence of a rare earth metal (Europium) as opposed to an organic fluorophore.

Recently Developed

HQ-O

A recently published study characterized this tracer’s ability to form a fluorescent precipitate with zinc and in doing so giving a high contrast and resolution staining of amyloid plaques. The staining time is of intermediate length, typically between 1 and 24 hours, depending on the temperature of the stain and the nature of prior tissue processing.

Upcoming

Amylo-Glo ® II

This tracer, like the original Amylo-Glo, will stain both extracellular amyloid plaques and intracellular neurofibulary tangles in both AD human autopsy and transgenic mouse brain tissue. The primary difference between Amylo-Glo and Amylo-Glo II is that the former is available as a 10X solution containing NaOH (final pH = 10.5), while Amylo-Glo II is offered as a powder that can be reconstituted in a vehicle of 50% ethanol. Although both tracers exhibit essentially the same labeling pattern, the Amylo-Glo II is more stable in solution and results in labeling of noticeably higher contrast, as illustrated to the left.