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Fluoro-Ruby

Compound: Fluoro-Ruby

Classification: Tetramethylrhodamine dextran-amine, 10,000 molecular weight

Appearance: Pink to crimson colored powder

Solubility: Highly soluble in aqueous solutions

Purity: Thin layer chromatography using cellulose plates and a solvent system of acetonitrile and water (6:4) revealed no detectable free TRITC dye.

Substitution: 1 molecule TRITC per molecule of dextran-amine

Average Molecular Weight: 10,500

Excitation Peak: 540 nm

Emission peak: 600 nm

Filter system for visualizing: Standard TRITC filter

Storage: The dry powder can be stored at room temperature or refrigerated. Solutions should be refrigerated when not in use.

Toxicity: Although the compound appears to be of low toxicity, it has not been extensively evaluated and therefore routine laboratory caution should be exercised. Not approved for human consumption.

REFERENCE:
Schmued, L., Kyriakidis, K., & Heimer, L., In vivo anterograde and retrograde axonal transport of the fluorescent rhodamine-dextran-amine, Fluoro-Ruby, within the CNS. Brain Res, 526 (1990) 127-134.